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KMID : 0364820080440010010
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Korean Journal of Microbiology
2008 Volume.44 No. 1 p.10 ~ p.13
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Degradation of Endocrine Disrupting Chemicals by Laccase Transformant of Phlebia
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Yeo Su-Min
Choi Hyoung-Tae
Kim Myung-Kil
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Abstract
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Endocrine disrupting chemicals (EDCs) are hard to be degraded in nature, and are also accumulated in diverse
organisms. They finally give negative effects to human through the food web. White rot fungi which have lignin-
degrading enzymes have high potentials for degradation of recalcitrant compounds, and a white rot fungus,
Phlebia tremellosa, isolated in Korea show good degrading activity against the endocrine disrupting phthalates.
We have isolated a laccase cDNA which was involved in the degradation of EDCs, and constructed a laccase
expression vector to use in the genetic transformation of P. tremellosa. The expression vector was stably integrated
into the chromosomal DNAs and showed increased laccase activity in transformants. One of transformants
showed not only increased degradation of several EDCs but also faster estrogenic decreasing activities
generated by the EDCs.
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KEYWORD
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endocrine disrupting chemicals, estrogenic activity, laccase expression, Phlebia tremellosa
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